Acetic Acid HPLC Assay Kit

Sucrose phosphorylase (SP) (EC2.4.1.7) mainly exists in microorganisms and plants. Sucrose phosphorylase cleaves glycosidic bonds, catalyzes the transfer of glucose into fructose, xylose, galactose and rhamnose, and synthesizes the corresponding glucose-based oligosaccharides. In addition, sucrose phosphorylase can also catalyze the synthesis of arbutin from hydroquinone, which has a strong whitening effect and has important applications in the cosmetics industry. BC0455 -- Page 2 / 4 Sucrose phosphorylase can use phosphate as an acceptor to catalyze the production of glucose 1. β-phosphate in sucrose is converted into glucose 6-phosphate under the catalysis of glucose phosphomutase, and is converted into glucose 6-phosphate. NADP+ is reduced to NADPH under the action of phosphate dehydrogenase. This results in increased light absorption at 340nm. Sucrose phosphorylase activity can be calculated by measuring the absorbance increase rate at 340 nm.